Capacitive Hyperthermia as an Alternative to Brachytherapy in Treatment of Human Prostate Cancer Cell

Introduction: The aim of this study was the evaluation of induced DNA damages of human prostate cancer cells, DU-145, treated with a combination of radiofrequency capacitive hyperthermia (HT) and teletherapy (EBRT) compared to a combination of teletherapy with high dose rate brachytherapy (BR).   Materials and Methods: DU-145 cells were cultured as spheroids in 300-micron diameter. Then the following treatments were conducted: a) EBRT at doses of either 2Gy or 4Gy of photon 15MV, b) HT for 30, 60, and 90 minutes’ duration at 43°C from a 13.56MHz radiofrequency capacitive heating device (Celsius TCS), c) BR with Ir-192 seed at doses of either 2Gy or 5.5Gy, d) The mentioned HT followed by EBRT (HT+EBRT) and e) EBRT followed by BR (EBRT+BR). Alkaline comet assay was performed to measure tail moment. Results: The induced DNA damages of DU-145 cells treated by adding HT to EBRT compared with EBRT alone, showed a significant enhancement; 3.28 and 5.14 times respectively for 30 and 60 minutes HT. By plotting dose-response curves, we could find a range of doses, which create radiobiological iso-effect in HT+EBRT and EBRT+BR treatments. Conclusion: This study suggests that about DNA damages of DU-145 cells, HT+EBRT could partly be considered as an alternative to EBRT+BR.